Coding

Part:BBa_K1998008:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


psbELJ-TB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 67
    Illegal AgeI site found at 173
    Illegal AgeI site found at 457
    Illegal AgeI site found at 466
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genes were codon optimised for bacteria. Lac promoter at the start of the operons. RBS are present before the start of each gene. Double terminator present at the end of the operon.

Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References